Journal:

Article Title: Endothelial Cell Prostaglandin I 2 and Platelet-Activating Factor Production Are Markedly Attenuated in the Calcium-Independent Phospholipase A 2 ? Knockout Mouse †

doi: 10.1021/bi100752u

Figure Lengend Snippet: Lung endothelial cell cultures isolated from wild type (left panel) and knockout (right panel) mice. Cultures were stained with rabbit antifactor VIII antibody followed by goat antirabbit Alexa Fluor 568 (red) and with DAPI (blue) to localize cell nuclei.

Article Snippet: After incubation in blocking solution (1% albumin and normal goat serum in PBS) with rabbit antifactor VIII antibody (AbCam, Cambridge, MA), cultures were washed and treated with Alexa Fluor 568 goat antirabbit IgG (Molecular Probes, Eugene, OR).

Techniques: Isolation, Knock-Out, Staining